FIG. 1.

(A) Bronx box. The Polaroid film cassette is placed over the microtiter plate containing the LRP-infected mycobacterial cultures. The door is closed, and the overnight film exposure begins. (B) Drug susceptibility test result gained from the Bronx box. Strains H37RV, AWC, and 14EBS were incubated for 72 h in media supplemented with INH, RIF, STR, and EMB at the concentrations shown (in micrograms per milliliter). The cultures were infected with phAE142, incubated for 8 h at 37°C, when luciferin buffer was added, followed by overnight exposure to the Polaroid film. The lower row shows the controls without antibiotic. The well labeled pKB15 corresponds to M. bovis BCG::pKB15, which acts as a reagent control. The absence of signal indicates susceptibility to all antibiotics in H37Rv. Strain AWC shows resistance to INH and STR, while strain 14EBS resists all antibiotics.