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. 2003 Oct;41(10):4865–4869. doi: 10.1128/JCM.41.10.4865-4869.2003

FIG. 2.

FIG. 2.

Standardization of the Bronx box assay. (A) Light signals obtained after infection of BCG Pasteur with phAE85 (left) and phAE142 (right) with the same multiplicity of infection. (B) Standardization of the phAE142 infection time prior to luciferin addition. Time points (hours) are indicated next to the corresponding signal. Film was exposed overnight after substrate addition. (C) Shown are the light signals observed after 2 (left) and 14 (right) h of exposure to the Polaroid film (after the addition of luciferin to an 8-h phAE142-infected culture).