TABLE 1.
Restriction endonucleases used in MLRFT
| Locus | Size of product (bp) | No. of MLST alleles/locusa | Restriction enzyme(s) | No. of MLRFT alleles/locus |
|---|---|---|---|---|
| arcC | 570 | 37 | HinfI | 3 |
| aroE | 536b | 66 | AluI and CfoI | 5 |
| glpF | 543c | 44 | Tsp509I | 9 |
| gmk | 488 | 35 | CfoI | 5 |
| pta | 575 | 44 | RsaI | 7 |
| tpi | 475 | 58 | BbuI and MboI | 4 |
| yqiL | 598 | 52 | VspI and DdeI | 4 |
a
The MLST allele count from the MLST database (http://www.mlst.net) as of February 3, 2003.
b
The aroE amplification product contains an AluI restriction site between the 3′ end of the trimmed MLST sequences and the aroE-Dn primer-binding site. This AluI site is polymorphic but is not used for allele differentiation to retain concordance with the MLST database.
c
The glpF amplification product contains two nonpolymorphic Tsp509I restriction sites between the primer-binding sites and the trimmed MLST sequence, one at each end.