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Recombinant dynamitin inhibits MPR transport from endosomes to the TGN. Purified recombinant dynamitin was added to reactions containing 1 mg/ml cytosol. Transport is given relative to control reactions containing equal amounts of BSA. Cytosol-dependent transport was obtained by subtracting transport measured in the absence of cytosol. Shown are average and SDs of two independent experiments carried out in duplicate.
