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. 1999 Jul;10(7):2199–2208. doi: 10.1091/mbc.10.7.2199

Figure 1.

Figure 1

Pulsed field gel analysis of amplified sod2 DNA. Undigested (U) or NotI-digested (N) total chromosomal DNA samples from wild-type and two representative amplified strains were separated by CHEF gel electrophoresis. (A) Ethidium bromide–stained gel. (B) Southern transfer probed with a sod2 probe. (C) Southern transfer probed with telomere probe. The lanes marked 225 and 180 contain DNA from a strain carrying the 225- or 180-kb amplicon, respectively; wt, wild-type; L, NotI restriction fragment L, which contains the endogenous sod2 gene; 225 and 180, amplified sod2 DNA. S. cerevisiae chromosomes in agarose plugs (Life Technologies, Bethesda, MD) were used as DNA size markers (left) and are given in kilobases.