Skip to main content
. 1999 Jul;10(7):2199–2208. doi: 10.1091/mbc.10.7.2199

Figure 2.

Figure 2

Topology of amplified sod2 DNA. (A) Total chromosomal DNAs from S. cerevisiae (Sc) and an S. pombe LiCl-resistant strain with the 225-kb amplicon (225) were treated (+) or mock-treated (−) with either exonuclease III (lanes 1–4) or topoisomerase I (lanes 5–8) and analyzed by CHEF electrophoresis. (B) Effect of ethidium bromide on electrophoretic mobility. Total chromosomal DNAs of S. cerevisiae (Sc), an S. pombe LiCl-resistant strain with the 225-kb amplicon (225), and a cosmid (cos) were separated on CHEF gels in the presence or absence of ethidium bromide (0.05 μg/ml). DNA size markers (left) are in kilobases.