Figure 4.
LC folding requirements for H-L assembly and CH1 domain folding. COS cells were transfected with cDNAs for the indicated genes. After 3 h of labeling, cell lysates were prepared in the presence of NEM and apyrase and then reacted with the indicated antibodies or protein A-Sepharose alone (P.A.). Immunoprecipitated proteins were analyzed by SDS-PAGE under nonreducing conditions. The mobilities of the various folding intermediates of both the heavy and light chain are shown to the right.