Figure 6.

Mapping of the importin β binding domain of SREBP-2. (A) Deletion mutants of SREBP-2 used in this study. All mutants were expressed as His-tagged GFP fusion protein. (B) Binding activity of SREBP-2 deletion mutants to importin β. E. coli lysate expressing each His-GFP-SREBP-2 deletion mutant was incubated with GST (G) or GST-importin β (β), and proteins bound to glutathione beads were analyzed by an anti-penta His antibody as in Figure 4C. E. coli lysate (L) expressing the indicated deletion mutant was directly loaded onto the gel.