TABLE 1.

Nucleotide sequences of primers and probes used in this study^a

Primer or probe and target	Name	Sequence (5′ → 3′)	Function	Positions	Tm (°C)	Refer- ence
Primers
stx1	KS7	CCC GGA TCC ATG AAA AAA ACA TTA TTA ATA GC	Sense	1120-1142	54.0	30
	KS8	CCC GAA TCC AGC TAT TCT GAG TCA ACG	Antisense	1401-1384	52.0	30
	StxA1 598	AGT CGT ACG GGG ATG CAG ATA AAT	Sense	598-621	56.9	2
	StxA1 1015	CCG GAC ACA TAG AAG GAA ACT CAT	Antisense	1015-992	55.3	2
stx2	LP43	ATC CTA TTC CCG GGA GTT TAC G	Sense	295-316	57.0	8
	LP44	GCG TCA TCG TAT ACA CAG GAG C	Antisense	881-860	57.0	8
	StxA2 679	TTC CGG AAT GCA AAT CAG TC	Sense	679-698	52.5	2
	StxA2 942	CGA TAC TCC GGA AGC ACA TTG	Antisense	942-922	54.6	2
Probes
stx1	StxA1 FL 724	CTG TCA CAG TAA CAA ACC GTA ACA TCG CTC-X	FL probe	724-695	65.5	2
	StxA1 LC 693 R7	LC-TGC CAC AGA CTG CGT CAG TGA GGT-ph	Red 705 probe	693-670	67.5	2
stx2	StxA2 FL 769	CAG AGC AGT TCT GCG TTT TGT CAC TGT CA-X	FL probe	769-797	65.0	2
	StxA2 LC 799 R6	LC-AGC AGA AGC CTT ACG CTT CAG GC-ph	Red 640 probe	799-821	63.3	2

^aSequence M19473 (14) was used as a reference for nucleotide positions of stx₁-specific primers and probes, and sequence X07865 (13) was used as a reference for all stx₂-specific oligonucleotides. T_m, melting temperature. X, 3′ fluoresceine; LC, 5′ LightCycler dye; ph, 3′ phosphate. FL, fluoresceine Red 640, LightCycler dye Red 640; Red 705, LightCycler dye Red 705.