Figure 7.

Effects of inositol starvation on cell viability. Wild-type (CTY182; closed circles), Δsac1 (CTY244; closed squares), Δsac1, Δcki1 (CTY; stippled cicles), and ino1-13 (CTY; open squares) strains were grown in minimal defined medium supplemented with inositol, harvested, washed, and shifted to inositol-free medium at time zero. Samples of each culture were subsequently removed at the indicated times after shift and serially diluted in YPD medium, and the serial dilutions were immediately plated onto YPD agar plates. Individual colonies were scored after 3 d of growth at 26°C to assess viable cell counts. Data are from a representative experiment.