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. 1999 Jul;10(7):2251–2264. doi: 10.1091/mbc.10.7.2251

Figure 3.

Figure 3

AtVTI1b but not AtVTI1a could replace yeast Vti1p in ALP and API traffic to the vacuole, which are transported to the vacuole via two different biosynthetic pathways. (A) Wild-type and vti1-2 cells (FvMY24) alone (−) or expressing either AtVTI1a or AtVTI1b were grown at 24°C, preincubated at 36°C for 15 min, pulse labeled for 7 min, and chased for 0 or 30 min at 36°C. ALP was immunoprecipitated from cellular extracts and separated by SDS-PAGE. The accumulation of pALP in vti1-2 cells was suppressed by expression of AtVTI1b but not by AtVTI1a. (B) Wild-type and vti1-11 cells (FvMY21) alone (−) or expressing AtVTI1a or AtVTI1b were grown at 24°C, preincubated at 36°C for 15 min, labeled for 10 min, and chased for 0 or 120 min at 36°C. API was immunoprecipitated from cellular extracts and analyzed by SDS-PAGE. Vacuolar mAPI was found only in vti1-11 cells expressing AtVTI1b, not in vti1-11 cells expressing AtVTI1a.