Figure 9.

T7-AtVTI1a and AtPEP12p colocalize on the PVC in cryosections of Arabidopsis roots from T7-AtVTI1a transgenic plants. (A and B) Ultrathin sections were incubated with T7 mAb followed by rabbit anti-mouse IgG and biotinylated goat anti-rabbit secondary antibody and were visualized with streptavidin conjugated to 10-nm colloidal gold. After the second fixation step (see MATERIALS AND METHODS), the same sections were incubated with AtPEP12p antiserum, followed by biotinylated goat anti-rabbit IgG, and then detected by streptavidin conjugated to 5-nm gold particles. (C) Control section. The same procedures were used as in A and B, except first antibodies were substituted with 2% BSA in PBS for T7 mAb and AtPEP12p preimmune serum for AtPEP12p antiserum. G, Golgi; arrow, AtVTI1a; arrowhead, AtPEP12p; bar, 0.1 μm.