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Effect of cytochalasin D on fluid phase endocytosis of HRP
| HRP uptake (ng/h per 106 cells) | |
|---|---|
| Control | 8.91 ± 2.1 |
| Cytochalasin D | 4.98 ± 1.3 |
Cells were preincubated with or without 10 μg/ml cytD for 30 min in DMEM. After the preincubation, the cells were incubated with HRP for 1 h at 37°C, and the cell-associated HRP activity was determined as described in MATERIALS AND METHODS. Data represent the values of three independent experiments in duplicate ± SEM.
