Figure 4.

Actin cytoskeleton organization in sla2Δ cells and cellular localization of the Sla2 deletion mutant proteins. The yeast strains are those described in Figure 3, A and B, with all plasmids being low copy number (CEN), except for pDD368, which is a high-copy-number (2 μ) plasmid expressing Sla2Δ33–359. Cells were grown at 25°C and were then fixed and stained with either anti-actin antibodies, or anti-Sla2p antibodies, as indicated. Cells expressing Sla2pΔ360–968, Sla2pΔ501–968, and Sla2pΔ33–359,576^stop were stained with the N-terminal Sla2p antibody. The remaining cells were stained with the C-terminal Sla2p antibody.