Figure 6.

Sla2p two-hybrid interactions. Yeast strain Y190 containing the indicated plasmids was replica plated either onto medium containing histidine (His⁺ plates), or onto medium lacking histidine and containing 3-aminotriazole (His⁻/3AT plates). If the DNA-binding domain fusion protein and DNA activation domain fusion protein interact with each other, the cells are able to grow on media lacking histidine. The Snf1p-Snf4p pair serves as a positive control. Plates were scored for growth after 2–3 d of growth at 25°C. Positive interactions were also verified by detection of lacZ expression (our unpublished results).