Fig. 2.
Representative microsphere center of mass (in x and y) with respect to the trap center during the unbinding assay. The Δd from the unloaded baseline position was measured and multiplied by the trap stiffness to obtain Funb. Inset: superimposed microsphere positions while the trap was pulled toward the upper right direction. The microsphere remained at the same position while the trap was pulled away, showing a displacement with respect to the trap. When actin was released from myosin, the bead sprang back to its unloaded baseline position.