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Total smooth muscle myosin heavy chain (SMMHC) and (+)insert isoform content of purified phasic [chicken gizzard (CG)] and tonic (bovine aorta) smooth muscle as assessed by Western blot analysis. Arrows indicate 200 kDa. Twenty-five and 12.5 ng of total protein were loaded for the chicken gizzard, whereas 1.2 and 0.6 μg of total protein were loaded for the aorta.
