Table 5.
Proteins of which abundance in DRM fractions changed in response to dDAVP as quantified by LC-MS/MS
| Protein Name; Gene Symbol | Abundance in DRMs | n | P | RefSeq Accession No. |
|---|---|---|---|---|
| Myosin 4; Myh4 | 1.0 ± 0.3 | 6 | 0.022 | NP_062198.1 |
| Keratin 15; Krt15 | 0.9 ± 0.3 | 4 | 0.064 | NP_001004022.1 |
| Keratin 19; Krt19 | 0.6 ± 0.2 | 6 | 0.042 | NP_955792.1 |
| Flotillin-2 (Reggie-1, REG-1); Flot2 | 0.4 ± 0.2 | 5 | 0.089 | NP_114018.1 |
| Keratin 12; Krt12 | 0.3 ± 0.1 | 10 | 0.022 | NP_001008761.1 |
| α-Actin cardiac; Actc1 | 0.3 ± 0.2 | 24 | 0.089 | NP_062056.1 |
| Keratin 75; Krt75 | 0.3 ± 0.1 | 35 | 0.038 | NP_001008828.1 |
| AQP1 (aquaporin-CHIP); AQP1 | 0.3 ± 0.1 | 3 | 0.025 | NP_036910.1 |
| Ras-related protein Rab7; Rab7 | 0.3 ± 0.1 | 3 | 0.055 | NP_076440.1 |
| Annexin A2; Anxa2 | 0.2 ± 0.1 | 24 | 0.066 | NP_063970.1 |
| Keratin 14; Krt14 | 0.2 ± 0.1 | 26 | 0.045 | NP_001008751.1 |
| β-Actin cytoplasmic; Actb | −0.2 ± 0.1 | 12 | 0.033 | NP_112406.1 |
| Prohibitin; Phb | −0.2 ± 0.1 | 5 | 0.059 | NP_114039.1 |
| Keratin 10; Krt10 | −0.2 ± 0.1 | 58 | 0.023 | NP_001008804.1 |
| Annexin A1; Anxa1 | −0.3 ± 0.1 | 6 | 0.001 | NP_037036.1 |
Values are means ± SE, expressed as log2 relative peptide abundance identified in DRM fractions (fractions 4-6) of vehicle- and dDAVP-treated IMCD cells; n, number of peptide elution profiles. P values are results of t-test to determine whether a given protein identification showed an increase or a decrease in association with the DRM fraction after dDAVP treatment. Several proteins did not show significant changes: AQP2, ATP synthase mitochondrial F1 complex α-subunit, ATP synthase mitochondrial F1 complex β-subunit, ATPase V1 complex B2 subunit, ATPase H+-transporting V0 complex subunit d1, ATPase Na+-K+-transporting α1-subunit, B-cell receptor-associated protein 37, crystallin αB, cytochrome c oxidase subunit IV isoform 1, cytochrome c oxidase subunit Va, heat shock-related 70-kDa protein 2, keratin 4, keratin 8, vesicle-associated membrane protein 2, syntaxin 7, and similar to SPFH domain family member 2. LC-MS/MS, tandem mass spectrometry.