Table 1.

Gene-specific primers used for real-time PCR analysis of hRFC, hPCFT, mRFC, mPCFT, and β-actin in Caco-2 cells and mice

Gene	Forward and Reverse Primers (5′-3′)	Fragment Size, bp
hRFC	CACCGACTAQCCTGCGCTACA	130
	GCCATGGTGACGCTGTAGAA
hPCFT	ATGCAGCTTTCTGCTTTGGT	100
	GGAGCCACATAGAGCTGGAC
mRFC	CGCATGCTAAGTGAACTGGTG	130
	TCAGTGCTTCTCCACAGGACAT
mPCFT	CTCATGTTCACAGGGTACGGATT	115
	ACAGCAGAGAACAGAGCACCCT
β-actin	CATCCTGCGTCTGGACCT	116
	TAATGTCACGCACGATTTCC

hRFC, human reduced folate carrier; hPCFT, human proton-coupled folate transporter; mRFC, mouse RFC; mPCFT, mouse PCFT.