FIG. 8.

Effect of Gα_o siRNA treatment on Noc-mediated Ca²⁺ current inhibition in SG neurons. Time courses of Ca²⁺ current amplitude inhibition acquired from Noc (3 μM) and NE (10 μM) application in a control (A) and a Gα_o siRNA-treated neuron (B). The currents were evoked every 10 s by a single 70-ms test pulse to +10 mV from a holding potential of −80 mV (shown Fig. 3A, top). ▪, application of Noc. Right: superimposed Ca²⁺ currents indicate the effect of Noc or NE prior to (bottom, a and c) and during (top, b and d) agonist application. C: summary graph showing the mean ± SE Ca²⁺ current inhibition produced by Noc and NE in control and siRNA-treated neurons. Numbers in parenthesis indicate the number of experiments.