Fig. 3.
MyD88−/− mice have a marked reduction in neutrophil recruitment after I/R. Mice were subjected to 30 min ischemia and 24 h of reperfusion. A: representative photomicrographs (×400) of myocardial sections labeled with hematoxylin and eosin (H&E) stain and anti-Gr-1 mAb (immunoperoxidase method). B: myeloperoxidase (MPO) activity (units × 10−2/mg; n = 8). MPO values for sham-operated hearts were 0.46 ± 0.03 for WT and 0.33 ± 0.07 units × 10−2/mg for MyD88−/− mice. P = 0.14 (not significant).