Fig. 5.

Bucillamine attenuates the expression of the pathological fetal gene program following I/R injury. Ventricular tissue was analyzed by ribonuclease protection assay for expression of pathological marker gene mRNAs as described in materials and methods. Individual values for each mRNA species were normalized to the mean sham value (saline or bucillamine, as appropriate) for the same mRNA. Each panel indicated the individual mRNA species measured, as follows: β-myosin heavy chain (β-MHC; A); atrial natriuretic peptide (ANP; B); skeletal α-actin (C); α-MHC (D); α-MHC-to-β-MHC ratio (E); and sarco(endo)plasmic reticulum Ca²⁺ ATPase 2a (SERCA2a; F).