Fig. 2.

Evaluation of peptide combinations from different protein families. Two peptides from three different protein families were combined serially in the proliferation assay, and the efficiency of the various peptide combinations was evaluated after calculating the isobolograms and C.I. for each of the combinations. (A) The isobolograms for the combination of a CXC chemokine-derived peptide and a TSP1 repeat-derived peptide. For each of the tested conditions (the individual peptides and the peptides tested in combination), proliferation dose response sigmoidal curves were estimated by fitting the data to sigmoidal Hill curves. Equally effective dose pairs in the combination experiments were then matched to the same level of inhibition obtained with a single peptide. The red highlighted areas designate peptide concentrations at which the activity is synergistic. The isobolograms for the combination of a collagen IV-derived peptide and a CXC chemokine and for a collagen IV-derived peptide and a TSP1 repeat-containing protein-derived peptide are included in Fig. S5. (B) Graphical interpretation of the most potent synergistic strategies, based on the calculations from the combinatorial experiments. The red lines indicate strong synergism and the blue lines antagonism.