Fig. 2.

Analysis of inducing structure over a large area of the 182-nt luciferase mRNA target. Partial sequence of mRNA target is shown (A) with the relative position of each individual pre-annealed 2′OMe ON A–F. The target sequence of the AON and siRNA guide strand is indicated in all capital letters. Each induced structure of A was used in cleavage assays for 30 s in the presence of 2.5 μM AON (B) or for 8.5 min in the presence of 2.5 μM siRNA (C) and analyzed on 10% sequencing gels. Negative controls (−) have no siRNA or AON, and positive controls (+) are cleavage reactions in the absence of 2′OMe ON.