Fig. 2.
Plots of stability and [NHopen] as a function of residue number. (A) The estimated stability (kcl/kop) for each amide. For highly protected amides with pH-independent proton occupancies, kcl/kop values are assigned a lower limit of 100. (B) [NHopen] values, estimated from kcl, kop, and tf. The red circles and blue triangles represent [NHopen] at tf of 0.4 and 6 ms, respectively. [NHopen] for highly protected amides is assigned a limiting value of 0. The location of the helices in the holomyoglobin structure is indicated at the top.