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. 2008 Sep 10;105(37):13889–13894. doi: 10.1073/pnas.0709135105

Fig. 5.

Fig. 5.

Function is maintained in Pim-WT animals after TAC. (A) Kaplan–Meier survival curve of NTG and Pim-WT animals following TAC or sham operation (NTG sham n = 6, Pim-WT sham n = 5, NTG TAC n = 8, Pim-WT TAC n = 8; **, P < 0.01 vs. Pim-WT TAC). (B) Histogram depicting heart weight/body weight ratios of NTG and Pim-WT sham and TAC hearts 4 weeks after the procedure (NTG sham n = 3, NTG TAC n = 4, Pim-WT sham n = 4, Pim-WT TAC n = 6; **, P < 0.01). (C) qRT–PCR analysis of NTG and Pim-WT hearts 4 weeks after TAC banding for molecular markers of hypertrophy (n = 4, *, P < 0.05, **, P < 0.01 vs. sham; ψ, P < 0.01 vs. NTG sham). (D) Histogram representing the number of TUNEL positive myocytes per mm2 in NTG and Pim-WT hearts after banding (n = 3, **, P < 0.01). (E) Masson trichrome staining of NTG and Pim-WT paraffin sections 4 weeks after banding and quantitation of collagen. (F) Quantitation of necrotic nuclei in NTG and Pim-WT animals pre- and post-TAC banding **, P < 0.01. (G) Analysis of antiapoptotic protein expression in NTG and Pim-WT whole heart lysates post-TAC. Quantitation of results for Bcl-xl, Bcl-2, and phosphorylated Bad (pBAD) levels in NTG and Pim-WT hearts. GAPDH was used as a loading control. n = 3, *, P < 0.05, **, P < 0.01 vs. GAPDH control. (G) Quantitation of Pim-WT hearts compared to NTG hearts post-TAC banding.