Maximum-likelihood putative gene tree based on the complete Prestin coding sequence of 22 mammals. Values indicate statistical support (maximum likelihood, maximum parsimony, neighbor-joining, and posterior probability, respectively). A filled circle indicates 100% support across methods and a negative sign (−) indicates lack of support for a specific method. Audiograms are given on the basis of published data (for references see SI Materials and Methods). For each plot, the x-axis ranges from 10 to 180 kHz, with ticks at 50, 100, 150, and 200, and the y-axis ranges from −20 to 100 dB with increments of 20 dB. The Yinpterochiroptera comprise the CF bats (green), Megaderma (orange), and the Old World fruit bats (blue), and both Yangochiroptera species are red.