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Characterization of RIP-Aire tg mice. (A) Histological analysis of pancreatic islets. Hematoxylin and eosin staining of 4 week-old pancreatic sections, 10× objective. Arrowheads point to individual islets. The pancreas from the RIP-Aire 22 mouse was fixed in Bouin's solution, resulting in darker staining. (B) RT-PCR analysis of Aire expression in whole pancreas from RIP-Aire mice (whole thymus RNA from a non-tg mouse as a reference). All values are mean ± SD when applicable.
