Fig. 2.

Endothelial Dicer inactivation reduces VEGF-induced angiogenesis. Ad-VEGF or Ad-eGFP was injected intradermally into the left and right ears, respectively, of 8-week-old mice. Frozen sections were stained for PECAM-1 (red) and counterstained with DAPI (blue). (A) Representative PECAM-1 staining showing reduced VEGF-induced angiogenesis in Tie2-Cre;Dicer^flox/flox mice compared with Dicer^flox/flox control mice. (B) Quantification of PECAM-1-positive structures (n = 6 per group). In B, for each animal, two to four images from five sections were quantified. Values are means ± SEM. *, P < 0.05, compared with Dicer^flox/flox by Student's t test. (Scale bars: 100 μm.)