Fig. 4.

Axonal development of newborn granule cells with DISC1 knockdown in the adult hippocampus. (A) A sample confocal projection image of mossy fiber axons (green) of newborn neurons with DISC1 knockdown at 6 wpi and DAPI staining (gray). Note the presence of GFP⁺ axons in the CA1 subfield. (Scale bars, 50 μm.) (B) Knockdown of endogenous full-length DISC1 by shRNA-D1. Primary hippocampal neurons were infected with lentivirus expressing shRNA-D1 and GFP (shRNA-D1), or GFP alone (control), at 2 days in vitro and analyzed at 9 days in vitro by Western blot (IB) using anti-DISC1 antibodies. The membrane was reblotted for GAPDH as a loading control. (C) Quantitative comparison of the length of the farthest axon from WT and shDISC1 mossy fibers at 1 and 2 wpi. Axons with DISC1 knockdown exhibit accelerated outgrowth compared with WT. **P < 0.01; *P < 0.05, Kolmogorov–Smirnov test.