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. Author manuscript; available in PMC: 2009 Sep 15.
Published in final edited form as: Anal Biochem. 2008 May 20;380(2):164–173. doi: 10.1016/j.ab.2008.05.018

Figure 5.

Figure 5

Real-time PCR amplification of thrombin-dependent ligation reactions. (A) Ct differences as a function of thrombin concentration. Ct differences were calculated relative to BSA alone (0.002% final). (B) Specificity of response. Ligation reactions were carried out in the presence of either 100nM thrombin or similar proteases, such as factor Xa and IXa. Either BSA or no protein controls were also carried out. Error bars were derived from at least three determinations.