Table 1.
Integrin subunit expression in ECV304 cells and HUVECs
| Integrin subunit | % Maximal mean fluorescence
|
|
|---|---|---|
| ECV304 | HUVEC | |
| β1 | 1.00 | 1.00 |
| αvβ5 | 0.40 | 0.27 |
| α2 | 0.39 | 0.59 |
| α3 | 1.40 | 0.40 |
| α4 | 0.10 | 0.09 |
| α5 | 0.22 | 0.68 |
| αv | 0.52 | 0.32 |
Cells (0.5 × 106) were incubated with primary antibodies, washed, incubated with an anti-mouse IgG conjugated to R-phycoerythrin, fixed, and analyzed for fluorescence using a flow cytometer. The relative fluorescence intensity is expressed as a percentage of β1 fluorescence.