Table 1.
Integrin subunit | % Maximal mean fluorescence
|
|
---|---|---|
ECV304 | HUVEC | |
β1 | 1.00 | 1.00 |
αvβ5 | 0.40 | 0.27 |
α2 | 0.39 | 0.59 |
α3 | 1.40 | 0.40 |
α4 | 0.10 | 0.09 |
α5 | 0.22 | 0.68 |
αv | 0.52 | 0.32 |
Cells (0.5 × 106) were incubated with primary antibodies, washed, incubated with an anti-mouse IgG conjugated to R-phycoerythrin, fixed, and analyzed for fluorescence using a flow cytometer. The relative fluorescence intensity is expressed as a percentage of β1 fluorescence.