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. 1998 Aug;9(8):1969–1980. doi: 10.1091/mbc.9.8.1969

Table 1.

Integrin subunit expression in ECV304 cells and HUVECs

Integrin subunit % Maximal mean fluorescence
ECV304 HUVEC
β1 1.00 1.00
αvβ5 0.40 0.27
α2 0.39 0.59
α3 1.40 0.40
α4 0.10 0.09
α5 0.22 0.68
αv 0.52 0.32

Cells (0.5 × 106) were incubated with primary antibodies, washed, incubated with an anti-mouse IgG conjugated to R-phycoerythrin, fixed, and analyzed for fluorescence using a flow cytometer. The relative fluorescence intensity is expressed as a percentage of β1 fluorescence.