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(A) Construct outline. (B) Adhesion assay. PH domain constructs of Btk do not inhibit Jurkat cell adhesion to ICAM-1. (C) Cellular fractionation. The btk motif is required for membrane association of the Btk-PH domain. (D facing page) Confocal laser scans according to Figure 3C. The PH domain and btk motif are simultaneously required for membrane association.
