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Two different pools of IκB can be detected in CHO cells. Cells were radiolabeled and subjected to immunoprecipitation with anti-IκB antibody as described in Figure 7, with the exception that radiolabeling was shortened to 2 h. (A) Quantification of levels of IκB immunoprecipitated during 40 min of chase in three different experiments. (B) Quantification of levels of IκB immunoprecipitated during 6–30 h of chase in three different experiments.
