Figure 1.

Molecular cloning of the unc-11 locus. (A) Genetic map of chromosome I of C. elegans. (B) Transgenic rescue of unc-11 mutants with DNA clones. Rescue data are provided as a fraction of stable lines that gave complete rescue of the unc-11 phenotype. (C) Organization of the unc-11 locus. A restriction map of the genomic unc-11 locus. Selected restriction sites are indicated on the linear map. Triangles delineate the positions of Tc1 transposons inserted in the unc-11 alleles listed in italics. Sequences deleted in unc-11 alleles are shown as hatched rectangles. Exons are represented as boxes. The structure of constructs used in this study are illustrated below the restriction map. A schematic representation of the six classes of unc-11 cDNAs is also shown. Exons are numbered 1–9. The a, b, or c suffixes represent alternative versions of these exons. GenBank accession numbers for the six cDNA forms are AF144257–AF144262.