Figure 1.

(A) Schematic representation of gar2 protein and its derivatives used in this study. Hatched box, N-terminal basic/acidic serine-rich domain; light gray boxes, RBDs containing conserved RNP1 motifs (checkered boxes); vertical-hatched box, GAR domain; black box, HA epitope; *, Y306, Y308, F409, and Y411 residues have been replaced with leucines. (B) Effects of gar2 mutations on cell growth. Growth comparisons were done by spotting each strain onto solid minimal medium after growing for 24 h in liquid medium at 30°C under derepressive conditions. The plates were incubated for 8 d at 19°C. From left to right, 25000, 5000, 1000, 200, and 40 cells were spotted.