Figure 5.

gar2 and gar2ΔRBDs migrations in glycerol velocity gradients. Equivalent amounts of total extracts from Sp91 (gar2-null) overexpressing gar2–HA or gar2ΔRBDs–HA were loaded onto 10–30% glycerol gradients and subjected to centrifugation. Both proteins were detected with anti-HA antibodies. Whereas gar2 belongs to 40–60S sedimentation coefficient complexes, gar2ΔRBDs protein is found either free at the top of the gradient or associated with structures larger than those with which the wild-type protein is associated.