Table 6.
Total cell lysate PE-PLD activity
| Genotype | PE-PLD activity (pmol/min/mg protein) |
|---|---|
| ARF1 ARF2 spo14::URA3 ADE2a | 180 ± 15 |
| arf1::HIS3 ARF2 spo14::URA3 ADE2b | 182 ± 22 |
| ARF1 ARF2 spo14::URA3 ade2-101c | 292 ± 70 |
| ARF1 arf2::LEU2 spo14::URA3 ade2-101d | 295 ± 86 |
| ARF1 ARF2 spo14::LEU2 ADE2 + GAL-ARF1 2μ URA3e | 174 ± 11 |
| ARF1 ARF2 spo14::LEU2 ADE2 + GAL-arf1Q71L 2μ uRA3f | 168 ± 69 |
| ARF1 ARF2 spo14::LEU2 ADE2 + GAL-arf1N126I 2μ URA3g | 156 ± 18 |
Total cell lysates were prepared from mitotically dividing yeast in triplicate and assayed for ScPld2 activity as described in MATERIALS AND METHODS. SDs were calculated with PLD values obtained from individual experiments.
a
Strain Y1125.
b
Strain Y1127.
c
Strain Y1126.
d
Strain Y1128.
e
Strain Y1129.
f
Strain Y1130.
g
Strain Y1131.