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. 1999 Jul;10(7):2377–2391. doi: 10.1091/mbc.10.7.2377

Figure 3.

Figure 3

Characterization of synchronized wild-type and dam1-1 haploid strains after shift to the nonpermissive (37°C) temperature. (A) Flow cytometric analysis to measure DNA content, as in Figure 1. Asynch, asynchronously growing cells at 25°C; 0, 2.2, and 3.3, hours at 37°C after removal of α-factor, %LB, percentage of cells with bud size more than half the size of the mother cell; 200–400 cells were counted. (B) Immunofluorescence staining of DNA (DAPI) and tubulin (α-Tubulin) in wild-type and dam1-1 cells grown at 37°C for 2.2 h (a and b) or 3.3 h (c). Of spindles from large-budded dam1-1 cells with two separated DAPI-staining regions at 2.2 h, 90% are discontinuous or abnormally thin, as in b and c, 6% are otherwise abnormal (e.g., not associated with DNA), and 4% appear normal (n = 200).