Figure 1.

Cloning zen-4. (A) Recombination data from three-factor mapping placed zen-4 11/28 of the distance between unc-44(e1260) and bli-6(sc16) on LGIV. zen-4 complements lag-1(q385) and pat-8(st554) but fails to complement the deficiency nDf41. (B) All zen-4 phenotypes are rescued by germline transformation with the cosmid M03D4 and the 7.0-kb XhoI–KpnI subclone pBR938. (C) zen-4 is oriented 5′ to 3′; exons are boxed; exon 7 contains an internal splice site resulting in the formation of two products. The w35 allele contains the insertion GATTT, which is predicted to result in six frameshifted amino acids and a premature stop codon following Arg 91 (asterisk).