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. 1998 Aug;9(8):2037–2049. doi: 10.1091/mbc.9.8.2037

Figure 2.

Figure 2

zen-4 encodes a member of the MKLP1 subfamily of kinesin-like proteins. Because of alternative splicing, zen-4 is predicted to encode a 775-amino acid protein (ZEN-4a) and a 772-amino acid (ZEN-4b) protein. (A) The predicted sequence of ZEN-4a. The putative nucleotide-binding motifs, N1, N2, and N3, are boxed; the putative microtubule binding sites, DLL and L12, are underlined. The location of the frameshift in the w35 allele is marked with an asterisk. (B) ZEN-4 belongs to the MKLP1 subfamily of kinesin-like proteins. The tree shown was constructed by Moore and Endow (1996) and is based on sequence alignment of the conserved kinesin motor domain. An updated version of the kinesin tree can be found at: http://www.blocks.fhcrc.org/∼kinesin/. (C) ZEN-4 sequence conservation with MKLP1 extends beyond the motor domain. A domain unique to the MKLP1 subfamily is 33% identical in ZEN-4 and MKLP1. While the coiled-coil domain is not well conserved, it contains the heptad repeats characteristic of a coiled coil. The complete sequence data for ZEN-4a and ZEN-4b are available from GenBank/EMBL/DDBJ under accession numbers AF057567 and AF057568.