Figure 2.

Microtubule morphologies of kip2Δ and kip3Δ mutants. The wild-type strain, MS1554 (A–C), the kip2Δ strain, MS2309 (D–L), and the kip3Δ strain, MS4516 (M–U), were grown to early exponential phase at 14°C. Cells were fixed in formaldehyde and stained with anti-tubulin YOL1/34 antibody (A, D, G, J, M, P, and S). Cells were also stained with DAPI to visualize nuclear material (B, E, H, K, N, Q, and T). Visualization of cells by Nomarski optics is seen in panels C, F, I, L, O, R, and U.