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. 1998 Aug;9(8):2051–2068. doi: 10.1091/mbc.9.8.2051

Table 5.

Viability of double mutants in combination with kip2Δ or kip3Δ

Mutant combination Tetrads analyzed No. of predicted mutants % of mutants dead or forming microcolonies Viability of double or triple mutant
 1. kip2Δ kar9Δ 33 33 100 SL
 2. kip2Δ dhc1Δ 33 31 6 viable
 3. kip2Δ jnm1Δ 11 10 0 viable
 4. kip2Δ act5Δ 12 13 0 viable
 5. kip2Δ bik1Δ 10 11 0 viablea
 6. kip2Δ cin8Δ 21 18 100b SL
 7. kip2Δ kip1Δ — — — viablec
 8. kip2Δ kar3Δ — — — viablec
 9. kip2Δ smy1Δ 8 7 0 viable
10. kip3Δ kar9Δ 29 23 12 viable
11. kip3Δ dhc1Δ 16 15 100 SL
12. kip3Δ jnm1Δ 29 29 100 SL
13. kip3Δ act5Δ 13 9 100 SL
14. kip3Δ bik1Δ 13 12 100 SL
15. kip3Δ cin8Δ 18 24 75d SL
16. kip3Δ kip1Δ 9 9 0 viable
17. kip3Δ kar3Δ 17 13 100 SL
18. kip3Δ kip2Δ 17 14 0 viable
19. kip2Δ dhc1Δ kar9Δ 18 8 100 SL
20. kip3Δ dhc1Δ kar9Δ 12 5 100 SL
21. kip2Δ kip3Δ kar9Δ 54 19 100 SL

Standard meiotic crosses were used to construct the indicated double mutants. The size of colonies was scored 2-3 d after germination. SL indicates that synthetic lethality was observed. The following crosses were carried out to create the indicated double and triple mutants: 1. kip2Δ kar9Δ, MS2310 × MS4263; 2. kip2Δ dhc1Δ, MS2354 × MS4304; 3. kip2Δ jnm1Δ, MS2310 × MS4321; 4. kip2Δ act5Δ, MS4308 × MS4586; 5. kip2Δ bik1Δ, MS4308 × MS4734; 6. kip2Δ cin8Δ, MS2310 × MAY2059; 9. kip2Δ smy1Δ, APY4ΔD5 × MS2354; 10. kip3Δ kar9Δ, MS4516 × MS4316; 11. kip3Δ dhc1Δ, MS4516 × MS4600; 12. kip3Δ jnm1Δ, MS4692 × MS4516; 13. kip3Δ act5Δ, MS4691 × MS4701; 14. kip3Δ bik1Δ, MS4691 × MS4915; 15. kip3Δ cin8Δ, MS4516 × MAY2058; 16. kip3Δ kip1Δ, MS4516 × MS2305; 17. kip3Δ kar3Δ, MS4516 × MS660; 18. kip3Δ kip2Δ, MS2442 × MS4691; 19. kip2Δ dhc1Δ kar9Δ, MS5214 × MS4062; 20. kip3Δ dhc1Δ kar9Δ, MS4654 × MS4304; and 21. kip2Δ kip3Δ kar9Δ, MS4308 × MS4648. 

a

Identical results were observed when kip2Δ was crossed to a bik1Δ in a nonisogenic strain (our unpublished observations). 

b

The viability of cin8Δ single mutants in this cross was 52%. This double mutant was also examined by plasmid loss assay on 5-FOA using a KIP2 URA3 CEN plasmid, which demonstrated that the cin8Δ kip2Δ double mutant lost a KIP2 CEN plasmid much less frequently than either wild-type or either single mutant. 

c

Data from Roof et al. (1992). 

d

The viability of cin8Δ single mutants in this cross was 67%.