Figure 5.
Effect of induction of lectin–GFP fusion proteins on amebic adherence to and killing of CHO cells. Amebae [uninduced or induced to express either 324 (A) or 224 (B) for 24 h] were incubated with CHO cells. An adherence assay was performed and the number of CHO cells adhered to each ameba was determined. (C) Inhibition of cytolysis of CHO cells on inducible expression of lectin–GFP fusion protein 224. Amebae (uninduced or induced to express 224 for 24 h) were incubated with 51Cr-labeled CHO cells and treated as in the adherence assay. Dextran was added to the amebae/CHO pellet to prevent further adherence events from occurring. The pellet was incubated at 37°C to allow for cytolysis of the CHO cells and the supernatant was removed and assayed for 51Cr release.