Figure 7.

Mutation of the lectin/integrin motif in the 224 fusion protein cytoplasmic tail abrogates the 224 phenotype. The cytoplasmic domain sequence in the 224 fusion protein was mutated to alanine at residue Y₁₂₆₁ (mut1) and at T₁₂₅₃, I₁₂₅₄, T ₁₂₅₅, and Y₁₂₆₁ (mut2). Adherence was measured in transfected amebae induced to express the 224, mut1, and mut2 fusion proteins for 24 h and compared with adherence of the uninduced amebae. For these experiments adherence was expressed as the percentage of adherence of the uninduced amebae, where an adherent ameba was counted as one containing at least three adherent CHO cells.