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Golgi association of Tlg1p as revealed by imunofluorescence microscopy. Cells expressing the myc-tagged Tlg1p were double labeled with 9E10 antibody (B) and polyclonal antibodies against endogenous Sed5p (C). Similarly, cells expressing myc-tagged Mnt1p were double labeled with 9E10 (E) and polyclonal antibodies against the endogenous Tlg1p (F). Also shown are the corresponding differential interference contrast images. Bar, 4 μm.
