Table 1.
Yeast strains used in this study
| Strain | Genotype | Source |
|---|---|---|
| Yeast | ||
| RSY255 | MATα ura3-52, leu2-3, 112 | R. Schekman |
| W303-D | MATa/α leu2-3,-112 ade2-1 ura3-1 his3-11,-15 trp1-1 can1-100 | U. Surana |
| JC20 | MATa Δtlg1::LEU2, GAL1-TLG1 ade2-1 ura3-1 his3-11,-15 leu2-3,-112 trp1-1 can1-100 | This study |
| JC21 | MATa Δtlg1::LEU2, GAL1-c-mycTLG1 ade2-1 ura3-1 his3-11,-15 leu-2-3,-112 trp1-1 can1-1-100 | This study |
| JC22 | same as W303-D with Δtlg1::LEU2/TLG1 | This study |
| ANY115 | MATa bet1-1 his4-619 ura3-52 | S. Ferro-Novick |
| SEY6210-sed5-1 | MATα leu2-3,112 ura3-52 his3-Δ200 trp1-Δ901 lys2-801 suc2-Δ9 sed5-1 | H. Pelham |
| RSY324 | MATa sec22-1 ura3-52 | S. Ferro-Novick |
| SEY6210 | MATa ura3-53 his3-Δ200 leu2-3,-112 trp1-Δ901 suc2-Δ9 lys2-801 | S. Emr |
| SEY6211 | MATα ura3-52 his2-Δ200 leu2-3,-112 trp1-Δ901 ade2-101 suc2-Δ9 lys2-801 | S. Emr |
| Y97 | RSY255 x SEY 6210 | This study |
| Y98 | MATa/α ade2-1, ade3Δ his3-11,-15 leu2-3,-112 trp1-Δ1 ura3-1 can1-100 | M. Cai |
| Y100 | SEY6210 X SEY6211 | This laboratory |
| FvMY7 | MATa ura3-52 his3-Δ200 leu2-3,-112 trp1-Δ901 suc2-Δ9 lys2-801 vti1-1 | T. Stevens |
| FvMY21 | MATa ura3-52 his3-Δ200 leu2-3,-112 trp1-Δ901 suc2-Δ9 lys2-801 vti1-11 | T. Stevens |
| RSY271 | MATa sec18-1 | R. Schekman |
| E. coli | ||
| GM119 | F′ supE44 lacY1 galK2 galT22 metB1 dam-3 dcm-6 tsx-78 λ- | ATCC |
| DH5α | F′ endA1 hsdR17 supE44 thil λ-recA gyrA96 relA1 (φ80 dlacZΔM15) | Hanahan |