Kinesin-related proteins in Dictyostelium. (A) The highly conserved P loop (IFAYGQT) and N-3 loop (LVDLAGSE) are underlined. The genes encoding the Dictyostelium KRPs have been mapped to YAC clones (Kuspa et al., 1992) and renamed according to Dictyostelium genetic nomenclature as follows: ksnB (K2), ksnC (K3), ksnD (K4), ksnF (K6), ksnG (K7), and ksnH (K8). The sequences have been submitted to GenBank and have the following accession numbers: K2 (AF015712), K3 (AF015714), K4 (AF015713), K6 (U69984), K7 (U41289), and K8 (U69985). (B) Expression patterns of Dictyostelium kinesins. KRP fragments were used to probe Northern blots of RNA from vegetative (T0) and developing (T8) cells. Exposure times varied from blot to blot. Transcripts are classified as “developmental” if they are expressed primarily in T8 cells and “constitutive” when comparable amounts are detectable at both time points.