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K7 is a plus end–directed motor. A polarity-labeled microtubule is shown in this fluorescence image to be moving from left to right, with the bright minus end of the microtubule leading. The triangle serves as a point of reference. Bar, 1 μm. Frames were taken approximately every 10 s, but velocities declined during prolonged fluorescence illumination; therefore, speed measurements were performed by imaging microtubules by video-enhanced differential interference contrast.
