Figure 5.

Immunodetection of K7 in Western blots. (A) Preadsorbed antiserum raised against a K7/MBP fusion protein was used to label a Western blot of total Dictyostelium proteins. The antiserum recognizes a band of ∼140 kDa (arrowhead) in proteins from cells that have been starved for 12 h (T12) but not in proteins expressed in vegetative cells (T0) or in K7 KO cells. Dashes indicate molecular mass standards of 205, 116, and 66 kDa, respectively. (B) Western blot of total proteins from T12 cells, and equivalent amounts of protein found in 38,000 × g supernatant (Sup) and pellet (Pell) fractions.